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Registro Completo |
Biblioteca(s): |
Embrapa Agricultura Digital. |
Data corrente: |
10/02/2014 |
Data da última atualização: |
08/01/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MENDES, T. A. O.; LOBO, F. P.; RODRIGUES, T. S.; RODRIGUES-LUIZ, G. F.; ROCHA, W. D. da; FUJIWARA, R. T.; TEIXEIRA, S. M. R.; BARTHOLOMEU, D. C. |
Afiliação: |
UFMG; FRANCISCO PEREIRA LOBO, CNPTIA; Centro Federal de Educação Tecnológica de Minas Gerais; UFMG; UFPR; UFMG; UFMG; UFMG. |
Título: |
Repeat-enriched proteins are related to host cell invasion and immune evasion in parasitic protozoa. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Molecular Biology and Evolution, Chicago, v. 30, n. 4, p. 951-963, 2013. |
DOI: |
10.1093/molbev/mst001 |
Idioma: |
Inglês |
Conteúdo: |
Proteins containing repetitive amino acid domains are widespread in all life forms. In parasitic organisms, proteins containing repeats play important roles such as cell adhesion and invasion and immune evasion. Therefore, extracellular and intracellular parasites are expected to be under different selective pressures regarding the repetitive content in their genomes. Here, we investigated whether there is a bias in the repetitive content found in the predicted proteomes of 6 exclusively extracellular and 17 obligate intracellular protozoan parasites, as well as 4 free-living protists. We also attempted to correlate the results with the distinct ecological niches they occupy and with distinct protein functions. We found that intracellular parasites have higher repetitive content in their proteomes than do extracellular parasites and free-living protists. In intracellular parasites, these repetitive proteins are located mainly at the parasite surface or are secreted and are enriched in amino acids known to be part of N- and O-glycosylation sites. Furthermore, in intracellular parasites, the developmental stages that are able to invade host cells express a higher proportion of proteins with perfect repeats relative to other life cycle stages, and these proteins have molecular functions associated with cell invasion. In contrast, in extracellular parasites, degenerate repetitive motifs are enriched in proteins that are likely to play roles in evading host immune response. Altogether, our results support the hypothesis that both the ability to invade host cells and to escape the host immune response may have shaped the expansion and maintenance of perfect and degenerate repeats in the genomes of intra- and extracellular parasites. MenosProteins containing repetitive amino acid domains are widespread in all life forms. In parasitic organisms, proteins containing repeats play important roles such as cell adhesion and invasion and immune evasion. Therefore, extracellular and intracellular parasites are expected to be under different selective pressures regarding the repetitive content in their genomes. Here, we investigated whether there is a bias in the repetitive content found in the predicted proteomes of 6 exclusively extracellular and 17 obligate intracellular protozoan parasites, as well as 4 free-living protists. We also attempted to correlate the results with the distinct ecological niches they occupy and with distinct protein functions. We found that intracellular parasites have higher repetitive content in their proteomes than do extracellular parasites and free-living protists. In intracellular parasites, these repetitive proteins are located mainly at the parasite surface or are secreted and are enriched in amino acids known to be part of N- and O-glycosylation sites. Furthermore, in intracellular parasites, the developmental stages that are able to invade host cells express a higher proportion of proteins with perfect repeats relative to other life cycle stages, and these proteins have molecular functions associated with cell invasion. In contrast, in extracellular parasites, degenerate repetitive motifs are enriched in proteins that are likely to play roles in evading host immune response. Altog... Mostrar Tudo |
Palavras-Chave: |
Evasão imune; Evolução genômica; Invasão celular; Parasitas; Proteínas. |
Thesaurus Nal: |
Cell invasion; Evolution; Immune evasion; Parasites; Proteins; Protozoa. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02770naa a2200349 a 4500 001 1979314 005 2020-01-08 008 2013 bl uuuu u00u1 u #d 024 7 $a10.1093/molbev/mst001$2DOI 100 1 $aMENDES, T. A. O. 245 $aRepeat-enriched proteins are related to host cell invasion and immune evasion in parasitic protozoa.$h[electronic resource] 260 $c2013 520 $aProteins containing repetitive amino acid domains are widespread in all life forms. In parasitic organisms, proteins containing repeats play important roles such as cell adhesion and invasion and immune evasion. Therefore, extracellular and intracellular parasites are expected to be under different selective pressures regarding the repetitive content in their genomes. Here, we investigated whether there is a bias in the repetitive content found in the predicted proteomes of 6 exclusively extracellular and 17 obligate intracellular protozoan parasites, as well as 4 free-living protists. We also attempted to correlate the results with the distinct ecological niches they occupy and with distinct protein functions. We found that intracellular parasites have higher repetitive content in their proteomes than do extracellular parasites and free-living protists. In intracellular parasites, these repetitive proteins are located mainly at the parasite surface or are secreted and are enriched in amino acids known to be part of N- and O-glycosylation sites. Furthermore, in intracellular parasites, the developmental stages that are able to invade host cells express a higher proportion of proteins with perfect repeats relative to other life cycle stages, and these proteins have molecular functions associated with cell invasion. In contrast, in extracellular parasites, degenerate repetitive motifs are enriched in proteins that are likely to play roles in evading host immune response. Altogether, our results support the hypothesis that both the ability to invade host cells and to escape the host immune response may have shaped the expansion and maintenance of perfect and degenerate repeats in the genomes of intra- and extracellular parasites. 650 $aCell invasion 650 $aEvolution 650 $aImmune evasion 650 $aParasites 650 $aProteins 650 $aProtozoa 653 $aEvasão imune 653 $aEvolução genômica 653 $aInvasão celular 653 $aParasitas 653 $aProteínas 700 1 $aLOBO, F. P. 700 1 $aRODRIGUES, T. S. 700 1 $aRODRIGUES-LUIZ, G. F. 700 1 $aROCHA, W. D. da 700 1 $aFUJIWARA, R. T. 700 1 $aTEIXEIRA, S. M. R. 700 1 $aBARTHOLOMEU, D. C. 773 $tMolecular Biology and Evolution, Chicago$gv. 30, n. 4, p. 951-963, 2013.
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Embrapa Agricultura Digital (CNPTIA) |
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Biblioteca(s): |
Embrapa Clima Temperado. |
Data corrente: |
15/03/2022 |
Data da última atualização: |
15/03/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 4 |
Autoria: |
DORNELES, A. O. S.; PEREIRA, A. S.; SILVA, T. B. da; TANIGUCHI, M.; BORTOLIN, G. S.; CASTRO, C. M.; PEREIRA, A. da S.; REISSER JUNIOR, C.; AMARANTE, L. do; HAERTER, J. A.; DUTRA, L. F. |
Afiliação: |
ATHOS ODIN SEVERO DORNELES; ALINE SOARES PEREIRA; TALIS BASILIO DA SILVA; MARISA TANIGUCHI; GABRIEL STRECK BORTOLIN; CAROLINE MARQUES CASTRO, CPACT; ARIONE DA SILVA PEREIRA, CPACT; CARLOS REISSER JUNIOR, CPACT; LUCIANO DO AMARANTE; JANNI ANDRE HAERTER, CPACT; LEONARDO FERREIRA DUTRA, CPACT. |
Título: |
Responses of Solanum tuberosum L. to Water Deficit by Matric or Osmotic Induction. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Potato Research, v. 64, p. 515-534, 2021. |
DOI: |
https://doi.org/10.1007/s11540-020-09489-3 |
Idioma: |
Inglês |
Conteúdo: |
Toselectpotatogenotypestoleranttowaterdeficit,systemstosimulatethisstresshavebeen used. Polyethylene glycol (PEG) is the main osmotic agent used for this purpose, but it causes an excessively severe stress. However, it is difficult to carry out an experiment that aims to compare plant responses under water deficit by osmotic or matric induction, and, thus,fewstudiescomparethesestress-inducingmechanisms.Therefore,theobjectiveofthis studywas to compare the responses of Agata, BRS Clara,C2406-03andCota genotypes to water deficit in both induction methods (matric or osmotic). The tests were carried out in a greenhouse, one using hydroponics (osmotic induction) and the other in pots with soil (matric induction). In both tests, the application of stressful conditions occurred at the beginning of tuberization. Assessments of gas exchange and shoot temperature were made throughout the exposure to stress. Also, samples were collected from leaves for analysis of osmotic potential and leaves and tubers for analysis of metabolite content. At the end of the potatoplantcycle,thenumberandweightoftuberswereevaluated.Inbothstressconditions, there were significant reductions in photosynthesis and transpiration rate compared to the respective normal hydration conditions. In addition, indicators such as metabolite levels (proline and soluble sugars) were significantly altered in plants exposed to different stress inductions. These data, together with the significant limitations in the growth of stressed plants, indicate that the experimental models induce similar responses. However, the water deficit by osmotic induction was more severe for the potato plants when compared to stress due to matric induction, mainly affecting tuber production. Therefore, the water deficit osmotic induction model can be recommended for phenotyping tolerance to this stress, due to the hydroponic system inducing greater tuber production per plant under optimal cultivation conditions. MenosToselectpotatogenotypestoleranttowaterdeficit,systemstosimulatethisstresshavebeen used. Polyethylene glycol (PEG) is the main osmotic agent used for this purpose, but it causes an excessively severe stress. However, it is difficult to carry out an experiment that aims to compare plant responses under water deficit by osmotic or matric induction, and, thus,fewstudiescomparethesestress-inducingmechanisms.Therefore,theobjectiveofthis studywas to compare the responses of Agata, BRS Clara,C2406-03andCota genotypes to water deficit in both induction methods (matric or osmotic). The tests were carried out in a greenhouse, one using hydroponics (osmotic induction) and the other in pots with soil (matric induction). In both tests, the application of stressful conditions occurred at the beginning of tuberization. Assessments of gas exchange and shoot temperature were made throughout the exposure to stress. Also, samples were collected from leaves for analysis of osmotic potential and leaves and tubers for analysis of metabolite content. At the end of the potatoplantcycle,thenumberandweightoftuberswereevaluated.Inbothstressconditions, there were significant reductions in photosynthesis and transpiration rate compared to the respective normal hydration conditions. In addition, indicators such as metabolite levels (proline and soluble sugars) were significantly altered in plants exposed to different stress inductions. These data, together with the significant limitations in the growth of... Mostrar Tudo |
Thesagro: |
Batata; Genótipo; Recurso Hídrico; Stress. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/232554/1/Artigo-Responses-of-Solanum.pdf
|
Marc: |
LEADER 02835naa a2200301 a 4500 001 2140920 005 2022-03-15 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s11540-020-09489-3$2DOI 100 1 $aDORNELES, A. O. S. 245 $aResponses of Solanum tuberosum L. to Water Deficit by Matric or Osmotic Induction.$h[electronic resource] 260 $c2021 520 $aToselectpotatogenotypestoleranttowaterdeficit,systemstosimulatethisstresshavebeen used. Polyethylene glycol (PEG) is the main osmotic agent used for this purpose, but it causes an excessively severe stress. However, it is difficult to carry out an experiment that aims to compare plant responses under water deficit by osmotic or matric induction, and, thus,fewstudiescomparethesestress-inducingmechanisms.Therefore,theobjectiveofthis studywas to compare the responses of Agata, BRS Clara,C2406-03andCota genotypes to water deficit in both induction methods (matric or osmotic). The tests were carried out in a greenhouse, one using hydroponics (osmotic induction) and the other in pots with soil (matric induction). In both tests, the application of stressful conditions occurred at the beginning of tuberization. Assessments of gas exchange and shoot temperature were made throughout the exposure to stress. Also, samples were collected from leaves for analysis of osmotic potential and leaves and tubers for analysis of metabolite content. At the end of the potatoplantcycle,thenumberandweightoftuberswereevaluated.Inbothstressconditions, there were significant reductions in photosynthesis and transpiration rate compared to the respective normal hydration conditions. In addition, indicators such as metabolite levels (proline and soluble sugars) were significantly altered in plants exposed to different stress inductions. These data, together with the significant limitations in the growth of stressed plants, indicate that the experimental models induce similar responses. However, the water deficit by osmotic induction was more severe for the potato plants when compared to stress due to matric induction, mainly affecting tuber production. Therefore, the water deficit osmotic induction model can be recommended for phenotyping tolerance to this stress, due to the hydroponic system inducing greater tuber production per plant under optimal cultivation conditions. 650 $aBatata 650 $aGenótipo 650 $aRecurso Hídrico 650 $aStress 700 1 $aPEREIRA, A. S. 700 1 $aSILVA, T. B. da 700 1 $aTANIGUCHI, M. 700 1 $aBORTOLIN, G. S. 700 1 $aCASTRO, C. M. 700 1 $aPEREIRA, A. da S. 700 1 $aREISSER JUNIOR, C. 700 1 $aAMARANTE, L. do 700 1 $aHAERTER, J. A. 700 1 $aDUTRA, L. F. 773 $tPotato Research$gv. 64, p. 515-534, 2021.
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